| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.028241 |
| Chromosome: | chromosome 4 |
| Location: | 326828 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217903 | (1 of 1) IPR002711//IPR003615 - HNH endonuclease // HNH nuclease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAGCAGGGGAGGCGGAAATGGCAGGGTCATCGGCAGCGGCATCTCCG |
| Internal bar code: | GAATGCGCACCTTAGAGATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTGGTACAAGGCCTTCC |
| Suggested primer 2: | CCGCTCGTTTTCGTTGGTTT |