| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.028253 |
| Chromosome: | chromosome 14 |
| Location: | 2760246 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626350 | (1 of 1) PF04751 - Protein of unknown function (DUF615) (DUF615) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGTCCTACCTTGCTTCTTTTGAAAGCCCCGGTTGCGACGTTGGCCG |
| Internal bar code: | TCCCGCCTCTAGAGCTACCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2358 |
| LEAP-Seq percent confirming: | 97.1429 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAGGAGTGAGGGAGGTGC |
| Suggested primer 2: | CGCATCATCTTGGCCACTTG |