Insertion junction: CLIP2.028267_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.028267
Chromosome:chromosome 12
Location:3259187
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g499400 CYN2,CYN18-2,CYN18B (1 of 1) K12733 - peptidyl-prolyl cis-trans isomerase-like 1 (PPIL1); Cyclophilin 18-2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGGGCCTGAGTTGGCCATGGACAGGATGCCGGCGCCGGTGTGCTTGA
Internal bar code:AATCTATGCCTGAGCATAGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3516
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGCTCCAGGTCGCATTCA
Suggested primer 2:CGCAAAAGTCTGACAACGCA