Insertion junction: CLIP2.028271_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.028271
Chromosome:chromosome 13
Location:397706
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g564650 MRS5 Magnesium and cobalt transport protein; (1 of 3) 3.6.3.2 - Magnesium-importing ATPase / Mg(2+)-importing ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGACTTTGGCTCTGGCGCATACATTGAGGTCGACCTACGAGGAGGAAAGA
Internal bar code:GTATTGGATCTACAGAAGTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3333
LEAP-Seq percent confirming:5.55556
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:AAAACACCATCACCAACGCG
Suggested primer 2:CAATCAGACGTGCAACCAGC