| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.028290 |
| Chromosome: | chromosome 14 |
| Location: | 4084681 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633650 | PRP6 | (1 of 1) PTHR11246:SF1 - PRE-MRNA-PROCESSING FACTOR 6; Splicing factor, component of the U4/U6-U5 snRNP complex | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTCTTACCTCCAGCAGCGCCCGTTCCTTGGCGCCGTCCCCTAGCTCC |
| Internal bar code: | TCTAGGGTATAGTGTCGGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1158 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACCCTACACCCATCCCC |
| Suggested primer 2: | GTCACACACACACACACACG |