| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.028295 |
| Chromosome: | chromosome 2 |
| Location: | 7201576 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390801 | (1 of 1) PTHR11918//PTHR11918:SF77 - RADICAL SAM PROTEINS // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGGTCGCCGCCCTTGCTGAAGCGCAGCCGCAGCCGCTGCGTCTTGGC |
| Internal bar code: | AAAATGAAAGATATTCGTACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1569 |
| LEAP-Seq percent confirming: | 56.25 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAAGCACACCGGCGTTTG |
| Suggested primer 2: | CCGCGATCATGCACTTTCAG |