Insertion junction: CLIP2.028313_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.028313
Chromosome:chromosome 4
Location:2941371
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g224700 NRT1 (1 of 1) K14638 - solute carrier family 15 (peptide/histidine transporter), member 3/4 (SLC15A3_4, PHT); Nitrate transporter NRT1 family intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATACCGGTTTTTTTAGTCCTTACTCCGACCTTTGAAGTCTTTCCCATCG
Internal bar code:GTGGTGAACGACCAGTGTAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2525
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CACACCTCTTCTCCCACCAC
Suggested primer 2:GCGCTCTCAACTTTGCAACA