| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.028320 |
| Chromosome: | chromosome 16 |
| Location: | 5156888 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685250 | SUB11 | (1 of 5) PTHR10795//PTHR10795:SF157 - PROPROTEIN CONVERTASE SUBTILISIN/KEXIN // MEMBRANE-BOUND TRANSCRIPTION FACTOR PEPTIDASE, SITE 1; Subtilisin-like protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCTGCCGCCATGGCGAGCACTCCGAGGGTAAGCGCGCCAGGGATGCG |
| Internal bar code: | GTGTGCAATCGTTTATTGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4556 |
| LEAP-Seq percent confirming: | 93.4783 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACTCAGCCGACCCTATCA |
| Suggested primer 2: | GATGCCGTACTGATGCCGTA |