| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.028333 |
| Chromosome: | chromosome 5 |
| Location: | 1096602 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248050 | (1 of 1) IPR012889 - L-fucose isomerase, N-terminal-2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCTCTGCCCCCTCCTTCCAACTCAGGGATTTTAACTAGTCCATCTG |
| Internal bar code: | ACGGTTTCACGTTTCTTGAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1090 |
| LEAP-Seq percent confirming: | 87.931 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAAGCCCACCCAATCGAG |
| Suggested primer 2: | AGATGGGACTTCAGGCTTGC |