Insertion junction: CLIP2.028429_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.028429
Chromosome:chromosome 9
Location:4642861
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g401812 (1 of 1) PF00580//PF13086//PF13087 - UvrD/REP helicase N-terminal domain (UvrD-helicase) // AAA domain (AAA_11) // AAA domain (AAA_12) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCTCCTCATGCTGGACGCCGGGCTGCCTGACCCATTCGCGGCGGCACA
Internal bar code:CAACAAGTGCTGAGTTCGTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1318
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTGTCGTACGTCTTCGG
Suggested primer 2:CTTGCTTTCACACCTGTGCC