Insertion junction: CLIP2.028431_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.028431
Chromosome:chromosome 6
Location:2772894
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g271400 PGP3 (1 of 3) 3.1.3.18 - Phosphoglycolate phosphatase; Phosphoglycolate phosphatase/4-nitrophenylphosphatase CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTTTGACTGCGATGGCGTGCTGTGGCGAGGCAGTGAGATCATCCACAA
Internal bar code:ACTTCGGAATCAATCGACACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2941
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCTACCCCCTGTTTCCTT
Suggested primer 2:TGGCACAGGGGTAATTGGAC