Insertion junction: CLIP2.028439_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.028439
Chromosome:chromosome 17
Location:4474401
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g730950 KIN2-2,KHP1,FLA10 Kinesin-II Motor Protein; (1 of 2) K10394 - kinesin family member 3/17 (KIF3_17) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACGGAAGTGCGCGGACACGCTGCACTTTCATGATACCCCATGACCTGG
Internal bar code:GTGTCAGTCGGTGTCGACTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3358
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:GGAAGGTGTTGGGGATGAGG
Suggested primer 2:CAGAGCTAAACGCAATCGGC