| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.028458 |
| Chromosome: | chromosome 14 |
| Location: | 992537 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613850 | (1 of 1) K12585 - exosome complex exonuclease DIS3/RRP44 (DIS3, RRP44) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGTGGAGCGCTTTTGTCGATTCCTGGGTTGTCCGCCGTCGCCTGCCT |
| Internal bar code: | AACAGTAGTCATTTAAATTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCAGACCCCAAGTAAGG |
| Suggested primer 2: | TGACGGACTTGGTGAAGTGG |