| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.028472 |
| Chromosome: | chromosome 1 |
| Location: | 5296025 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037200 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAGGAAGGCGAGGCACGGTGGCCGGTGCCCGGCTGCCGGCTCCTGGCA |
| Internal bar code: | ATTAGCTCTTCGGAATCCATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2173 |
| LEAP-Seq percent confirming: | 82.2222 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCCCTAGGTGACCTTTTG |
| Suggested primer 2: | CTGCAGCGTGCTTTCCTTTT |