| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.028492 |
| Chromosome: | chromosome 8 |
| Location: | 3053101 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375350 | SDR12 | (1 of 2) K19329 - WW domain-containing oxidoreductase (WWOX); Short-chain dehydrogenase/reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACACACAAAGACGCACACACACACACACACACACACACACACACACA |
| Internal bar code: | CCTATAGCTCCTCGTGACGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2457 |
| LEAP-Seq percent confirming: | 65.8537 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCCCGCTACTCTAACCT |
| Suggested primer 2: | GCATCCCAAACCAACACCAC |