| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.028510 |
| Chromosome: | chromosome 1 |
| Location: | 868522 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800008 | (1 of 31) PF03732 - Retrotransposon gag protein (Retrotrans_gag) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGCTGCTGGGCTGCACGTGCGCCGGGGCTGCCATGTTGGCTTAGCTT |
| Internal bar code: | CTGCGGCCAAGCTCTCGAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2261 |
| LEAP-Seq percent confirming: | 55.4217 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCTATCTCCAAGCTCGG |
| Suggested primer 2: | CGATGTAGATGGCGTCGTGA |