Insertion junction: CLIP2.028521_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.028521
Chromosome:chromosome 8
Location:3481121
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g378750 CTAP7 Chloroplast translocon associated protein 7 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTGAAAAACAAAACTAGTTCAGACAAGAGGTGGGCGCTGCCTGCGCTG
Internal bar code:ACTAGGCGCCCGGGCGAGATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1173
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGCGTTTTTCACTTCTGC
Suggested primer 2:CGTTCATAACCCCGTGTGGA