Insertion junction: CLIP2.028526_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.028526
Chromosome:chromosome 16
Location:1485070
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g652850 ALG5,GTR4 Dolichyl-phosphate beta-glucosyltransferase; (1 of 1) 2.4.1.117 - Dolichyl-phosphate beta-glucosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCCCTTCCTTTTCCTTCCCTCCCCGTCCCTCCTCCTCCCATCCCTTCC
Internal bar code:GTTAACAAAGCGTCTTGTGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:588
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGGTGTTGGGCGCTTTAG
Suggested primer 2:GCGACTGTTTCCTTACGGGA