Insertion junction: CLIP2.028527_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.028527
Chromosome:chromosome 16
Location:889089
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g648100 (1 of 1) K05757 - actin related protein 2/3 complex, subunit 1A/1B (ARPC1A_B) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGAACGGGCGAACGGCGTGCGGCCACGACACCCAGGGAACCTCCATCA
Internal bar code:GAGCGTCACGATCGTTCGACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:83
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACACGAGTACGACACCCAA
Suggested primer 2:CTCCCTGCAACAGTTTTGGC