Insertion junction: CLIP2.028531_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.028531
Chromosome:chromosome 7
Location:2464065
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g329250 Putative metallochaperone; (1 of 1) PF02492//PF07683//PF13414 - CobW/HypB/UreG, nucleotide-binding domain (cobW) // Cobalamin synthesis protein cobW C-terminal domain (CobW_C) // TPR repeat (TPR_11) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGAGGAGGCTGGGCGGGCGCACGAGGAAGAGGAGTACGGCATCGGCAC
Internal bar code:TTTTCTTGGTGTTGCTTGTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:84
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCACTACCACCATCAGCACT
Suggested primer 2:GTGGTGCTGCTGAACAAGTG