| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.028552 |
| Chromosome: | chromosome 17 |
| Location: | 327568 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698200 | MMS21 | SUMO ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAACATACGCGGATCTCGTTTCTGGTCTCCAACACACACACGGCGCTGC |
| Internal bar code: | TGTGCCGCAGTTGCTCCCCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCTCATTGTATCCGGGGG |
| Suggested primer 2: | GGGTGATTAATGGGGTGGCA |