| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.028558 |
| Chromosome: | chromosome 2 |
| Location: | 5602579 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115450 | CYG47 | (1 of 5) IPR000104//IPR001054//IPR029787 - Antifreeze protein, type I // Adenylyl cyclase class-3/4/guanylyl cyclase // Nucleotide cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCCGCATGCACACACCTTGCCTTTGACGCTCAGGCCGCCGGTGGGCA |
| Internal bar code: | GTCGCTTATTGCTAGTCTAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1407 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTCCTGAACGGTGTGTA |
| Suggested primer 2: | ACCTCTTTCCCAAGGCGATG |