| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.028567 |
| Chromosome: | chromosome 1 |
| Location: | 3822091 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025000 | SSA12 | cilia-sensing, structure and/or assembly; (1 of 1) K16056 - calcium release-activated calcium channel protein 1 (ORAI1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCACAAGAGCAAAGCCAGCCAGGAGGGCGGACAGGCTGCTGACCGCC |
| Internal bar code: | TTATGACTAGCAGTATACTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5699 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAGTTCATGTGCGTGCCA |
| Suggested primer 2: | GTGCATGAACTCAGCCTCCT |