| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.028573 |
| Chromosome: | chromosome 12 |
| Location: | 9405932 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541300 | KATL4 | katanin like protein 4, similar to the catalytic subunit; (1 of 9) 3.6.4.3 - Microtubule-severing ATPase / Katanin | 3'UTR_intron |
| Cre12.g541350 | (1 of 14) PF00566 - Rab-GTPase-TBC domain (RabGAP-TBC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAACCTGGTCAGGCGACGCCCGTGCCCGCACTTTGCCTGACCGCCTCA |
| Internal bar code: | GCCTGGACTCATAGTAACCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2218 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGCTTATCCATCGAAGCC |
| Suggested primer 2: | CACATGCCCCACAAAAGCAA |