Insertion junction: CLIP2.028586_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.028586
Chromosome:chromosome 5
Location:426897
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g243801 FAP387 Flagellar Associated Protein 387 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCCCACACGTCGACCACTCCGCTCTCCATGAACGGCCCAAGGCCCCCC
Internal bar code:AATTTACGGTCCCACCGATGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:65
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGTGTGTGTGTGTGTG
Suggested primer 2:GGATAGGCTCGAGGTCCAGA