| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.028591 |
| Chromosome: | chromosome 14 |
| Location: | 3247817 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g801651 | (1 of 1) PF09945 - Predicted membrane protein (DUF2177) (DUF2177) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCCCCAGACCGTGTCGATGGCCGCAAGCGCCCATGTCCAGGGTGTGA |
| Internal bar code: | GATTGTAAGGAAGATCTAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1247 |
| LEAP-Seq percent confirming: | 18.5185 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCCCATTCCTTACACGT |
| Suggested primer 2: | CAAGGGTTAGAGTGCAGGCA |