Insertion junction: CLIP2.028614_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.028614
Chromosome:chromosome 11
Location:1215308
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467688 (1 of 1) 1.2.3.7 - Indole-3-acetaldehyde oxidase / Indoleacetaldehyde oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAATAATGTAGAAGACGGCAAGCTATCAATCAAACAAACGATCGTGCGG
Internal bar code:TCGGTTTTAGGATATTGGAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:333
LEAP-Seq percent confirming:63.6364
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGGCCATTCATGTGGAGA
Suggested primer 2:AGGGAGATGTGCTGGAGGAT