Insertion junction: CLIP2.028614_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.028614
Chromosome:chromosome 11
Location:1215310
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467688 (1 of 1) 1.2.3.7 - Indole-3-acetaldehyde oxidase / Indoleacetaldehyde oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTCTTGGGCTAATCCATAGTGTTTGAATTTGCATTTCGGCACCCTTAC
Internal bar code:TCGGTTTTAGGATATTGGAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:587
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGAGATGTGCTGGAGGAT
Suggested primer 2:GGTGGCCATTCATGTGGAGA