| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.028632 |
| Chromosome: | chromosome 2 |
| Location: | 4422239 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105900 | (1 of 1) 3.1.1.7 - Acetylcholinesterase / True cholinesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCAGTCCCCAGTTTCCGACGGTGCCGTTGGCGGCGCTGTCCTTGAGTT |
| Internal bar code: | TAAAGTAGGCTGTACTCTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3927 |
| LEAP-Seq percent confirming: | 93.3962 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 106 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGCCGGTGTTGAAAATT |
| Suggested primer 2: | ACAAGCATCCCCACCAGATG |