| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.028642 |
| Chromosome: | chromosome 12 |
| Location: | 2184247 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509250 | EYE2 | Eyespot assembly protein 2; (1 of 1) PTHR18929//PTHR18929:SF99 - PROTEIN DISULFIDE ISOMERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAATCTTCCGCTTGCCCTCCATACGGCCATACGCGCCTGCGGCCTCTC |
| Internal bar code: | ACGGTAGCTGTCTTGGCTTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2922 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCCTTACAGGCACCAAGC |
| Suggested primer 2: | GGGACATTCACAGGCTCACA |