| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.028658 |
| Chromosome: | chromosome 7 |
| Location: | 1351045 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322650 | CSA1 | Chlorophyceae-specific gene family A protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTCTACAGGCGGAGGGGGATGGCGCCTGCCGCCTTCGGTCTTGGGCTC |
| Internal bar code: | TAGAGGCACTACGCGGAGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2390 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACATGCAGCGAATGAAG |
| Suggested primer 2: | GCTCAGGGCTACGGTATGTC |