| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.028660 |
| Chromosome: | chromosome 6 |
| Location: | 2840994 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272100 | (1 of 1) K15463 - tRNA A64-2'-O-ribosylphosphate transferase [EC:2.4.2.-] (RIT1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAAGGGTTCGCAGAAACAATTAATGGCCCGCCCATAGGTGCGCTAGAA |
| Internal bar code: | AAAGTTTAAATACTTGGTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4144 |
| LEAP-Seq percent confirming: | 58.6207 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGATCGTCTTCTGCTGT |
| Suggested primer 2: | CGAGTCCTTCGTCACCTCAG |