Insertion junction: CLIP2.028671_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.028671
Chromosome:chromosome 10
Location:2113839
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g433100 OBGE1,OBGE Factor possibly involved in assembly of the ribosomal 50S assembly; (1 of 1) K03979 - GTP-binding protein (obg) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCTTTGCCCCCCCCCCCCCGGAAAACTTTATCACCGAGGTCGCCCTG
Internal bar code:GGGCCAAAAGGCTGGGCTGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:319
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGGCGCGCATAAAACATA
Suggested primer 2:CCCACTCATCCTCGCAAAGT