| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.028677 |
| Chromosome: | chromosome 9 |
| Location: | 1822958 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397900 | (1 of 1) PTHR21347:SF0 - CLEFT LIP AND PALATE TRANSMEMBRANE PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGTCCAGCGTTTCGTTCACCTGGCCCAGGGGCGAAAGGTGCGGGGCG |
| Internal bar code: | TTAATTTGTGTGCCGTTCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1675 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGGACTGCACACGTTGG |
| Suggested primer 2: | CTCGCCTACCATAACCGTCC |