Insertion junction: CLIP2.028684_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.028684
Chromosome:chromosome 9
Location:460126
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre18.g749247 (1 of 6) PTHR10527 - IMPORTIN BETA CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAACAAATGCACGGCGCCATCCGATACCTCCCACGGCTCCTTGTCTCTG
Internal bar code:CAGTACTTACCATCATTGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:2171
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAGGAGATGTTCAGCTGC
Suggested primer 2:ATCTGAACGCAATGGGCTCA