Insertion junction: CLIP2.028689_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.028689
Chromosome:chromosome 9
Location:929477
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g403000 PAP9 (1 of 3) K03514 - non-canonical poly(A) RNA polymerase PAPD5/7 (PAPD5_7, TRF4); Class-II RNA nucleotidyl transferase 9 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGACGTGTTTACACCATTCCCAATATGAAACCCGAGACACGAAAGCCGC
Internal bar code:CGCTGGGACGCTGTCGGTTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:110
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGACACTGTGCAACCGAC
Suggested primer 2:TTCATCTGCTCCTTGGCGTT