| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.028691 |
| Chromosome: | chromosome 5 |
| Location: | 645010 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800523 | (1 of 31) PF03732 - Retrotransposon gag protein (Retrotrans_gag) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTAGCGGTTTTCTTCCGCTGGCAGGGGGTCACCAGTTACAACGGCTC |
| Internal bar code: | TCTTGACCTTGCTACAATCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5202 |
| LEAP-Seq percent confirming: | 48.1481 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTCTTCATCGCGATGCAG |
| Suggested primer 2: | TTCCGGTTCCATCTCGGTTG |