Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | + |
Strain: | CLIP2.028703 |
Chromosome: | chromosome 14 |
Location: | 554588 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g611517 | (1 of 3) K07393 - putative glutathione S-transferase (ECM4) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGATCGGGTGGAGGGGTTGGGTGGAGAGGTCGGGTGGAGGGGTTGGG |
Internal bar code: | TTCTAATGGGGTAAAATTGAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 677 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 7 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCGGGGTAGTGATTTGGGG |
Suggested primer 2: | CGTTGTTGATCGCGCTGTAG |