| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.028749 |
| Chromosome: | chromosome 12 |
| Location: | 3907874 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801398 | (1 of 17) PF08016 - Polycystin cation channel (PKD_channel) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCCATGAGGCTGCCTGCCGGGAAGCCCGGCACTAGCAGCCTCAAGGCG |
| Internal bar code: | GTGTTAGGGCATGTCAGCTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3934 |
| LEAP-Seq percent confirming: | 86.5248 |
| LEAP-Seq n confirming: | 122 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 141 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAGGTACCAGGAGAGGA |
| Suggested primer 2: | ATAGCTAGGCACGTGCAGTC |