Insertion junction: CLIP2.028769_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.028769
Chromosome:chromosome 8
Location:3658456
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g379950 PFH17,P4H17,PHX12 Prolyl 4-hydroxylase 17; (1 of 2) PTHR10869//PTHR10869:SF79 - PROLYL 4-HYDROXYLASE ALPHA SUBUNIT // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGAACTGTGAGCAACAGTGAGCATGATCCTTTCCGGTGCATGCGCTCC
Internal bar code:CCGACCAATGACTTCGCTCTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1000
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AAACACACACACACCTCGGT
Suggested primer 2:CTGTGGGCTAGGGTTTCAGG