Insertion junction: CLIP2.028771_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.028771
Chromosome:chromosome 9
Location:5381879
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g408100 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCACAGAGCACGGAGGAGGTTTCGAACGGACTGCATGGGGTTGCAGCA
Internal bar code:TTCGATTGCAGCGCAACGATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:3418
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:93
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:93

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTGTGTGTCCATGTGC
Suggested primer 2:ACGCTCTTCACGCAACTGTA