Insertion junction: CLIP2.028798_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.028798
Chromosome:chromosome 6
Location:1513412
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g260700 XUV1,UAPA6 (1 of 2) K06901 - putative MFS transporter, AGZA family, xanthine/uracil permease (pbuG); Xanthine/uracil/vitamin C permease-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATACCGTAGATAGGGTCCGGCGCCAACCGAGGGAAGCGCACGCACGCAC
Internal bar code:GTTACCATACAAAGGTCACGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2404
LEAP-Seq percent confirming:2.63158
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCACGCTTCCTATCTAGT
Suggested primer 2:CAATCTGCGTGATCTTGCCG