Insertion junction: CLIP2.028801_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.028801
Chromosome:chromosome 6
Location:6068014
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g800713 (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTAGGTGACGCCACCGACTGTCATATACTGACACTCCTGCGAGTCCAG
Internal bar code:AACACTACCGTAAAGGCAAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:173
LEAP-Seq percent confirming:77.7778
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TTCACGACTACCTCTCCCCC
Suggested primer 2:TTGACGCTGATCATGGTGCT