Insertion junction: CLIP2.028809_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.028809
Chromosome:chromosome 8
Location:2268910
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g370700 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACACACGCGCGCACGTGGCCCCCCCCAGCCAACACGCAGTCCA
Internal bar code:TATCGATGATTGCGGAGTGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1275
LEAP-Seq percent confirming:96.4286
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCAGAGTACGCATACCAT
Suggested primer 2:CGCTCCACCTTGAACTCCAT