Insertion junction: CLIP2.028821_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.028821
Chromosome:chromosome 2
Location:5531898
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g114850 (1 of 1) PTHR22904:SF169 - RNA POLYMERASE II-ASSOCIATED PROTEIN 3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATTATGGGGGCTGCGCATGTGCTCTACTGCTTTACTGTAGTCATCGGGC
Internal bar code:TGGAAAACAGGTTGTAAAAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:517
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GACCCCAACGTACTGCTGAA
Suggested primer 2:TGTACGGTTGTCTGTCGGTG