Insertion junction: CLIP2.028855_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.028855
Chromosome:chromosome 13
Location:5102125
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g606800 (1 of 2) PTHR30336//PTHR30336:SF4 - INNER MEMBRANE PROTEIN, PROBABLE PERMEASE // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAATGCATACCGCAACACTCCGCGCCCGGAATGCCGCCCCGCGGGCGC
Internal bar code:CGGAGACCGGCGTGGGATGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2597
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCAGCCGTTTGACATCGT
Suggested primer 2:CCCCTCACCCAGTGATAGGA