Insertion junction: CLIP2.028898_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.028898
Chromosome:chromosome 2
Location:1621249
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g085150 (1 of 19) PTHR31251:SF1 - SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 10-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATCCAATCATCAATCAAGTCGTCGCAACCGGCGGCATGCATCGCCACG
Internal bar code:CAGCAATGCTTGGACTTTGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:855
LEAP-Seq percent confirming:4.34783
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CTAGTGCGCAACCCCAACTA
Suggested primer 2:TGTTGATTGTGCGTGCTGTG