| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.028925 |
| Chromosome: | chromosome 2 |
| Location: | 5527452 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g114800 | (1 of 1) PF14934 - Domain of unknown function (DUF4499) (DUF4499) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGGCAACGCCTGTCGGTGCGGCGGGGCAAAGGGGCGCCATGTGTGTA |
| Internal bar code: | GCAGAAGCTATGTGGTCTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3309 |
| LEAP-Seq percent confirming: | 89.2857 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCACCGCTTCCTGAAATC |
| Suggested primer 2: | CCATGGCTGTTGGTGTTTGG |