Insertion junction: CLIP2.028947_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.028947
Chromosome:chromosome 12
Location:3121800
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g500850 (1 of 85) IPR009057 - Homeodomain-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAGCACTACTCAACCGCCAAGGACACTCCGCAGGACCTTAGCCTGCGC
Internal bar code:CGGTCCGTCATCACGGCTTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1874
LEAP-Seq percent confirming:95.8333
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTCAGACGAGGATGACGC
Suggested primer 2:CGTGGATGTGACAAGGAGCT