Insertion junction: CLIP2.028953_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.028953
Chromosome:chromosome 2
Location:1993383
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g088250 MCT2 Malonyl-CoA:acyl-carrier-protein transacylase; (1 of 2) K00645 - [acyl-carrier-protein] S-malonyltransferase [EC:2.3.1.39] (fabD) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCACCACCCACCCGCAGCCCCTCTTCAAAGGTGAGTGCGCCCGCGAAC
Internal bar code:AAGACCCGATTGTCCTTGTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1603
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGACTAACCGCTGCCCTATT
Suggested primer 2:ATGTTTCTCTACCACGCGCA